Virtual Proteomics Laboratory

Virtual Proteomics Laboratory

An Overview of Virtual Proteomics Laboratory
An Overview of Proteomics and our Team Member
Module I: An Overview of Gel-based Proteomics
Module I: An Overview of Gel-based Proteomics
Experiment-1: Gel-based proteomics to analyze human serum proteome
Objective: To compare the serum protein expression profiles of diseased patients having fever and healthy control samples using 2-D gel electrophoresis.
Experiment-2: Gel-based proteomics to analyze bacterial proteome
Objective: To compare the profiles of drug treated and normal E. coli cultures grown under identical conditions using 2-D gel electrophoresis.
Experiment-3: Gel-based proteomics to analyze plant proteome
Objective: To compare the protein expression profiles of plant leaves grow under conditions of drought (stress) and normal conditions using 2-D gel electrophoresis.
Module II: An Overview of MALDI-TOF MS
Module II: An Overview of MALDI-TOF MS
Experiment-4: In-gel digestion of proteins for MS analysis
Objective: To excise the spots from 2D or SDS-PAGE gels and perform in-gel trypsin digestion
Experiment-5: Sample preparation for the MALDI-TOF MS analysis
Objective: To prepare the test and standard samples for the MALDI-TOF MS analysis
Experiment-6: MALDI-TOF instrumentation and analysis of serum proteins
Objective: MALDI-TOF instrumentation and generation of peptide mass fingerprinting (PMF)from the serum proteins
Experiment-7: MS data analysis - Peptide Mass Fingerprinting (PMF)
Objective: To analyze the MALDI-TOF peptide mass fingerprinting data
Experiment-8 : Molecular Weight Determination of Intact Protein Using MALDI TOF
Determining the molecular weight of intact protein using MALDI TOF
Module III: An Overview of Bioinformatics
Module III: An Overview of Bioinformatics
Experiment-9 : Sequence Alignment
To perform Sequence Alignment
Experiment-10 : Homology Modelling
Homology Modelling
Experiment-11 : Protein Function Annotation
Protein Function Annotation
Experiment-12 : Molecular Docking
Molecular Docking
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